Seaman Donald Duck, USN is the son of Hortense and Quackmore Duck, and the twin brother of Della Duck . Donald was raised by Elvira Coot (Grandma Duck) as an infant with his sister, Della Duck who is the mother of Huey, Dewey and Louie Duck. Whether he was returned to his parents or stayed with Grandma Duck was unknown, but sometime in his childhood, he first met his uncle Scrooge McDuck along with his parents, his sister Della, and his aunt Matilda. Sometime after Donald grew up, he became the legal guardian of his three nephews Huey, Dewey and Louie Duck. Sometimes, Donald works for his uncle Scrooge as his employee and works for him for as little as 30 cents an hour.

Donald currently dates Daisy Duck, who's affection he sometimes has to compete over with his cousin Gladstone Gander. Donald's other nemesis besides Gladstone is his next door neighbor known only as Neighbor Jones.

One of Donald's friends is the inventor Gyro Gearloose, and in his time away from Duckburg he was friends with Mickey Mouse, Goofy, Jose Carioca and the Panchito Pistoles. He was voiced by Tony Anselmo.